APC anti |
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Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
FC - Quality tested Recommended UsageEach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Excitation Laser Red Laser (633 nm) Application NotesThe M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1 Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656). Application References(PubMed link indicates BioLegend citation) Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP) Viville S, et al. 1993. Cell 72:635. (IHC) Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC) Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block) Yamashita I, et al. 1993. Int. Immunol. 5:1139. Guo M, et al. 1995. Zygote 3:65. (IHC) Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block) Luckashenak NA, et al. 2006. J. Immunol. 177:5177. Venanzi ES, et al. 2007. J. Immunol. 179:5693. Christensen SR, et al. 2006. Immunity 25:417. PubMed Matte-Martone C, et al. 2008. Blood 111:3884. PubMed De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed Kuns RD, et al. 2009. Blood 113:5999. PubMed Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed Fu H, et al. 2014. 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